Review



systems mab100502  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 92
      Buy from Supplier

    Structured Review

    R&D Systems systems mab100502
    Systems Mab100502, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/systems+mab100502/Human%2FPrimate+Ig+Kappa+Light+Chain+Antibody/pmc11688428-138-17-16
    Average 92 stars, based on 1 article reviews
    systems mab100502 - by Bioz Stars, 2026-09
    92/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Proteolysis targeting chimera (PROTAC)-driven antibody internalization of oncogenic cell surface receptors
    Article Snippet: Both, the dynamin I/II or clathrin inhibitors Dyngo-4a and Pitstop2 (Med Chem Express HY-13863 and HY-115604, respectively) and Uba1 inhibitors TAK-243 and PYZD-4409 (Med Chem Express HY-100487 and HY-13297, respectively) were added to the cells at the same time that labeled antibodies Tmab and anti-MET were added, and internalization evaluated by acquiring images with IncuCyte every 30 min for 24–48 h as before. .. Internalized antibodies were also tested by Western blots using primary antibodies detecting light and heavy chains (R&D Systems MAB100502 and MAB1101, respectively) derived from Human IgGs in BT-474 and SKBR3 cell lines treated with proteasome inhibitor MG132 (Selleckchem S2619). .. Cells were seeded at a density of 400,000 cells/well in 1.5 mL of culture medium in 6-well plates (Corning CLS3603) and incubated overnight at 37 °C before addition of antibody or PROTAC with antibody at different time points.

    Derivative Assay:

    Article Title: Proteolysis targeting chimera (PROTAC)-driven antibody internalization of oncogenic cell surface receptors
    Article Snippet: Both, the dynamin I/II or clathrin inhibitors Dyngo-4a and Pitstop2 (Med Chem Express HY-13863 and HY-115604, respectively) and Uba1 inhibitors TAK-243 and PYZD-4409 (Med Chem Express HY-100487 and HY-13297, respectively) were added to the cells at the same time that labeled antibodies Tmab and anti-MET were added, and internalization evaluated by acquiring images with IncuCyte every 30 min for 24–48 h as before. .. Internalized antibodies were also tested by Western blots using primary antibodies detecting light and heavy chains (R&D Systems MAB100502 and MAB1101, respectively) derived from Human IgGs in BT-474 and SKBR3 cell lines treated with proteasome inhibitor MG132 (Selleckchem S2619). .. Cells were seeded at a density of 400,000 cells/well in 1.5 mL of culture medium in 6-well plates (Corning CLS3603) and incubated overnight at 37 °C before addition of antibody or PROTAC with antibody at different time points.



    Similar Products

    systems mab100502  (R&D Systems)
    92
    R&D Systems systems mab100502
    Systems Mab100502, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/systems+mab100502/Human%2FPrimate+Ig+Kappa+Light+Chain+Antibody/pmc11688428-138-17-16
    Average 92 stars, based on 1 article reviews
    systems mab100502 - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

    Image Search Results